ELISA General RNA Extraction
General RNA Extraction Kit
Catalog No.: R1051 (50 preps)
Kit Contents
Component R1051
50 preps
Solution RL 60 mL
Wash Buffer RPI 18 mL
Wash Buffer RW 12 mL
DEPC-treated water 10 mL
RNase-free Spin Column 50 each
RNase-free Microcentrifµge Tube 50 each
Materials supplied by the users:
• Chloroform
• Ethanol (96–100%)
Description
General RNA Extraction Kit provides a simple method of isolating total RNA from a wide range of sample types and amounts. In general, samples are lysed and then homogenized in the presence of guanidinium isothiocyanate, a chaotropic salt which is capable of protecting the RNA from endogenous RNases. After homogenization, ethanol is added to the sample. The sample is then processed throµgh a spin column containing a clear silica-based membrane where the RNA binds. Any impurities are effectively removed by subsequent washing. The purified total RNA is then eluted in RNase-Free Water and is suitable for use in a variety of downstream applications.
Downstream Applications
Purified RNA is free of impurities and enzyme inhibitors, and have an A260/280=1.8-2.0, is suitable for applications such as:
• Reverse transcription PCR (RT-PCR)
• Real-time quantitative PCR (qPCR)
• Northern blotting
• Nuclease protection assays
• RNA amplification for microarray analysis
• cDNA library preparation after poly(A)+ selection
Features
• Stable yield
• Reliable performance of high-quality purified total RNA in downstream applications
Storage
DEPC-treated water store at -20 ̊C, other reagents store at 2-8 ̊C, protect from light. Kit contents are stable for up to 12 months when stored properly.