ELISA General RNA Extraction

General RNA Extraction Kit Catalog No.: R1051 (50 preps) Kit Contents Component R1051 50 preps Solution RL 60 mL Wash Buffer RPI 18 mL Wash Buffer RW 12 mL DEPC-treated water 10 mL RNase-free Spin Column 50 each RNase-free Microcentrifµge Tube 50 each Materials supplied by the users: • Chloroform • Ethanol (96–100%) Description General RNA Extraction Kit provides a simple method of isolating total RNA from a wide range of sample types and amounts. In general, samples are lysed and then homogenized in the presence of guanidinium isothiocyanate, a chaotropic salt which is capable of protecting the RNA from endogenous RNases. After homogenization, ethanol is added to the sample. The sample is then processed throµgh a spin column containing a clear silica-based membrane where the RNA binds. Any impurities are effectively removed by subsequent washing. The purified total RNA is then eluted in RNase-Free Water and is suitable for use in a variety of downstream applications. Downstream Applications Purified RNA is free of impurities and enzyme inhibitors, and have an A260/280=1.8-2.0, is suitable for applications such as: • Reverse transcription PCR (RT-PCR) • Real-time quantitative PCR (qPCR) • Northern blotting • Nuclease protection assays • RNA amplification for microarray analysis • cDNA library preparation after poly(A)+ selection Features • Stable yield • Reliable performance of high-quality purified total RNA in downstream applications Storage DEPC-treated water store at -20 ̊C, other reagents store at 2-8 ̊C, protect from light. Kit contents are stable for up to 12 months when stored properly.