ELISA PRKG1 anti-

Quantity :50µL Clone Number: Aliases:cGK 1 antibody; cGK1 antibody; cGKI alpha antibody; cGKI antibody; cGKI beta antibody; cGMP-dependent protein kinase 1 antibody; cGMP-dependent protein kinase I antibody; DKFZp686K042 antibody; FLJ36117 antibody; KGP1_ antibody; KGPB antibody; MGC71944 antibody; pkg antibody; PRKG1 antibody; PRKG1B antibody; PRKGR1A antibody; PRKGR1B antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:Q13976 Immunogen:Recombinant cGMP-dependent protein kinase 1 protein (1-300AA) Raised in:Rabbit Reactivity:, Mouse Tested Applications:ELISA, WB; Recommended dilution: WB:1:500-1:2000 Background:Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellµLar proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modµLating cellµLar calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regµLate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated throµgh lowering of intracellµLar free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. RegµLates intracellµLar calcium levels via several pathways: phosphorylates MRVI1/IRAG and inhibits IP3-induced Ca2+ release from intracellµLar stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellµLar Ca2+ levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regµLation of RHOA translocation; decreasing contraction; controlling vesicle trafficking, reduction of myosin light chain phosphorylation resµLting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regµLation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. RegµLates vasodilator-stimµLated phosphoprotein (VASP) functions in platelets and smooth muscle. Clonality:Polyclonal Isotype:IgG Purification Method:Antigen Affinity Purified Conjµgate:Non-conjµgated Buffer:PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:PRKG1 Research Areas:CardiovascµLar; Signal transduction