ELISA Gabra3 anti-
Quantity :100µL
Clone Number:
Aliases:Gabra3 antibody; Gabra-3 antibody; Gamma-aminobutyric acid receptor subunit alpha-3 antibody; GABA(A) receptor subunit alpha-3 antibody
Product Type:Polyclonal Antibody
Immunogen Species:Rattus norvegicus (Rat)
UniProt ID:P20236
Immunogen:Peptide sequence around aa. 33~37(R-R-Q-E-P)derived from Rat GABA A Receptor a3.
Raised in:Rabbit
Reactivity:, Rat, Mouse
Tested Applications:ELISA, WB; WB:1:500-1:1000
Background:GABA (g-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modµLate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modµLate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release throµgh suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit throµgh coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).
Jones, K.A. et al. (2000) Neuropsychopharmacology 23, S41-9.
Duthey, B. et al. (2002) J Biol Chem 277, 3236-41.
Kaupmann, K. et al. (1997) Nature 386, 239-46.
Clonality:Polyclonal
Isotype:IgG
Purification Method:Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjµgates. Antibodies were purified by affinity-chromatography using epitope-specific peptide
Conjµgate:Non-conjµgated
Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form:liquid
Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names:Gabra3
Research Areas:Neuroscience