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ELISA Galectin-8 (LGALS8)

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Reactivity: (Homo sapiens) UniProt:O00214 Abbreviation:LGALS8 Alternative Names:Gal-8; PCTA-1; PCTA1; Po66-CBP; Po66 carbohydrate binding protein|galectin 8|galectin-8|galectin-8g|prostate carcinoma tumor antigen 1 Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.118 ng/mL Intra-AssayCV:?3.8% Inter-AssayCV:?7.3% Recovery:0.86 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate LGALS8 in samples. An antibody specific for LGALS8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGALS8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LGALS8 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS8 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Galectin-8 is one of the galectins, beta-galactoside-binding lectins with conserved carbohydrate recognition domains (CRD). Galectin-8 essentially consists of two carbohydrate recognition domains separated by a linker that bears no similarity to any known protein. The gene symbol is LGALS8 Galectin-8 has been identified originally as PCTA1 [Prostate carcinoma tumor antigen 1], an antigen expressed by prostate cancer cells Several isoforms appear to be expressed differentially in various tumoral cells The protein is identical with Po66-CBP [Po66 carbohydrate-binding protein], identified by the Po66 monoclonal antibody. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).

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